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Image Search Results
Journal: BioMed Research International
Article Title: Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway
doi: 10.1155/2016/9014364
Figure Lengend Snippet: TM caused apoptotic alternations in mitochondrial function. (a) TM caused intracellular ROS accumulation in HepG2 and SMMC-7721 cells analyzing via DCFH-DA staining after 6 h incubation (20x; scale bar: 100 μ m) ( n = 3). (b) TM induced MMP dissipation detecting by JC-1 staining after 6 h incubation (20x; scale bar: 100 μ m) ( n = 3). (c) 24 h TM exposure enhanced the expressions of cleaved-PARP, Bax, and Bad and suppressed the levels of Bcl-2. Quantification data of protein expressions were normalized by corresponding GAPDH and the average fold of band intensity was marked, respectively ( n = 3).
Article Snippet: After three washes, fluorescent color changes were analyzed using fluorescent microscopy under Nikon
Techniques: Staining, Incubation